Electric road vehicles - overview, concepts and research at Reutlingen university

The paper details the architecture of fully electrified vehicles as well as their new electronic systems. Examples of up-to-date electrical passenger cars are given. A very important question, that is the environmental foot-print of electrical vehicles compared to conventional ones, is examined. A research project is introduced where a fleet of two-wheeled vehicles is available for day-to-day use. Research on vehicles, software for fleet management and battery range prediction is described.В данной статье привeдены подробные сведения о принципе работы электрифицированных транспортных средств, а также описаны их новые электрические системы. Показан примеры уже существующих электрических пассажирских транспортных средств. Рассмотрено влияние электрифицированного транспорта на окружающую среду в сравнении с обычными видами транспорта. Приведен проект исследований, в рамках которого для ежедневного использования существует парк двух колесных электрифицированных транспортных средств. Описаны исследования, непосредственно связанные с электрифицированным транспортом, определением точного времени разряда батареи, а также программным обеспечением, позволяющим управлять парком таких транспортных средств.У статті наведено докладні відомості щодо принципів роботи електрифікованих транспортних засобів, а також описано їх нові електричні системи. Показано приклади вже існуючих електричних пасажирських транспортних засобів. Розглянуто вплив електрифікованого транспорту на навколишнє середовище у порівнянні із звичайними видами транспорту. На- ведено проект досліджень, у рамках якого існує парк двоколісних електрифікованих транспортних засобів для щоденного використання. Описано дослідження, безпосередньо пов'язані із електрифікованим транспортом, визначенням точного часу розряду батареї, а також програмним забезпеченням, що дозволяє керувати парком таких транспортних засобів

From Writer to Teacher: The Gradual Release of Responsibility in an Early Childhood Education Writing Course for Pre-Service Teachers

Teaching students to become confident, capable writers is imperative in today’s world. Growing attention has been paid to the amount and kinds of writing students are experiencing in schools with an urgent plea for more time and attention given to writing instruction (Nagin, 2003; National Commission on Writing, 2003). Yet, few teachers feel well prepared to teach writing. In this special issue on writing methods courses, we discuss the evolution of our writing methods course for early childhood preservice teachers (PK-5). Specifically, we examine the current pedagogical practices within the course to support preservice teachers’ experiential learning. This piece examines how a gradual release of responsibility model with multiple units of study supports preservice teachers as writers and as future teachers of writers

Management system for building materials as a basis for closed loop material flow analysis considering material efficiency and climate change mitigation

Resource management is becoming increasingly important in the construction sector. In order to support the recycling of materials, it is necessary to determine the quantities in the building stock and those caused by construction activities. At present, a large number of different actors use different categories for construction materials and the raw materials they consist of as well as for waste categories, depending on their field of activity. This results in imprecisions that make it difficult to consistently track and influence mass flows and hinder targeted resource management. This is the starting point of this paper as it discusses possibilities to establish a consistent allocation of materials to context-typical groups following the approach of continuous material flow analysis. On the input-side, aspects of mineral planning and on the output-side aspects of waste and secondary raw material management are being considered and references to grey emissions are established along the entire process chain. In this way, cross-departmental planning relating to recycling management and climate protection will be supported. With regard to the object of consideration and the level of action, a distinction is made between two different spatial scale levels: on the one hand, the individual building level, where the material inventory approach is used to provide detailed information on the building\u27s material composition, and on the other hand the regional level, for which more aggregated information on building material groups is provided in the form of material cadastres. Current results of a research project in Germany are presented

Targeted online liquid chromatography electron capture dissociation mass spectrometry for the localization of sites of in vivo phosphorylation in human Sprouty2

We demonstrate a strategy employing collision-induced dissociation for phosphopeptide discovery, followed by targeted electron capture dissociation (ECD) for site localization. The high mass accuracy and low background noise of the ECD mass spectra allow facile sequencing of coeluting isobaric phosphopeptides, with up to two isobaric phosphopeptides sequenced from a single mass spectrum. In contrast to the previously described neutral loss of dependent ECD method, targeted ECD allows analysis of both phosphotyrosine peptides and lower abundance phosphopeptides. The approach was applied to phosphorylation analysis of human Sprouty2, a regulator of receptor tyrosine kinase signaling. Fifteen sites of phosphorylation were identified, 11 of which are novel

High-resolution maps of material stocks in buildings and infrastructures in Austria and Germany

The dynamics of societal material stocks such as buildings and infrastructures and their spatial patterns drive surging resource use and emissions. Two main types of data are currently used to map stocks, night-time lights (NTL) from Earth-observing (EO) satellites and cadastral information. We present an alternative approach for broad-scale material stock mapping based on freely available high-resolution EO imagery and OpenStreetMap data. Maps of built-up surface area, building height, and building types were derived from optical Sentinel-2 and radar Sentinel-1 satellite data to map patterns of material stocks for Austria and Germany. Using material intensity factors, we calculated the mass of different types of buildings and infrastructures, distinguishing eight types of materials, at 10 m spatial resolution. The total mass of buildings and infrastructures in 2018 amounted to ∼5 Gt in Austria and ∼38 Gt in Germany (AT: ∼540 t/cap, DE: ∼450 t/cap). Cross-checks with independent data sources at various scales suggested that the method may yield more complete results than other data sources but could not rule out possible overestimations. The method yields thematic differentiations not possible with NTL, avoids the use of costly cadastral data, and is suitable for mapping larger areas and tracing trends over time

Current challenges in software solutions for mass spectrometry-based quantitative proteomics

This work was in part supported by the PRIME-XS project, grant agreement number 262067, funded by the European Union seventh Framework Programme; The Netherlands Proteomics Centre, embedded in The Netherlands Genomics Initiative; The Netherlands Bioinformatics Centre; and the Centre for Biomedical Genetics (to S.C., B.B. and A.J.R.H); by NIH grants NCRR RR001614 and RR019934 (to the UCSF Mass Spectrometry Facility, director: A.L. Burlingame, P.B.); and by grants from the MRC, CR-UK, BBSRC and Barts and the London Charity (to P.C.

Signal Peptide-Dependent Inhibition of MHC Class I Heavy Chain Translation by Rhesus Cytomegalovirus

The US2-11 region of human and rhesus cytomegalovirus encodes a conserved family of glycoproteins that inhibit MHC-I assembly with viral peptides, thus preventing cytotoxic T cell recognition. Since HCMV lacking US2-11 is no longer able to block assembly and transport of MHC-I, we examined whether this is also observed for RhCMV lacking the corresponding region. Unexpectedly, recombinant RhCMV lacking US2-11 was still able to inhibit MHC-I expression in infected fibroblasts, suggesting the presence of an additional MHC-I evasion mechanism. Progressive deletion analysis of RhCMV-specific genomic regions revealed that MHC-I expression is fully restored upon additional deletion of rh178. The protein encoded by this RhCMV-specific open reading frame is anchored in the endoplasmic reticulum membrane. In the presence of rh178, RhCMV prevented MHC-I heavy chain (HC) expression, but did not inhibit mRNA transcription or association of HC mRNA with translating ribosomes. Proteasome inhibitors stabilized a HC degradation intermediate in the absence of rh178, but not in its presence, suggesting that rh178 prevents completion of HC translation. This interference was signal sequence-dependent since replacing the signal peptide with that of CD4 or murine HC rendered human HCs resistant to rh178. We have identified an inhibitor of antigen presentation encoded by rhesus cytomegalovirus unique in both its lack of homology to any other known protein and in its mechanism of action. By preventing signal sequence-dependent HC translocation, rh178 acts prior to US2, US3 and US11 which attack MHC-I proteins after protein synthesis is completed. Rh178 is the first viral protein known to interfere at this step of the MHC-I pathway, thus taking advantage of the conserved nature of HC leader peptides, and represents a new mechanism of translational interference